Traditional bisulfite based methyl conversion methods result in spurious fragmentation of cfDNA and double-stranded library preparation methods (dsPreps) lose critical information at cfDNA native termini.

When combined with SRSLY library preparation followed by enzymatic methyl-conversion - captures both fragmentomics and methylation signals along the entire cfDNA fragment. By enabling robust capture of cfDNA methylation patterns and fragmentomic features, SRSLY™ supports early cancer detection research and advanced epigenetic biomarker discovery.

Use our SRSLY MethylPlus kit with Methylated Adapters and Uracil-tolerant Polymerase for Methyl-Seq with cfDNA (250pg - 50ng) and other inputs where native termini retention is critical.

key data highlights