SRSLY® captures features of circulating cell-free DNA (cfDNA) to support high-complexity NGS library preparation. Its high sensitivity enables reliable measurement of low-input, highly fragmented cfDNA commonly found in liquid biopsy samples. By reducing measurement bias and sample handling variability, SRSLY helps improve consistency and confidence in cfDNA sequencing workflows.

With PicoPlus and NanoPlus Kits you can work with inputs as low as 250 pg to 50ng.

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SRSLY™ with ForShear™ enables robust NGS library preparation from challenging FFPE-derived DNA samples. The enzymatic fragmentation module, is optimized to accommodate degraded and chemically modified DNA commonly encountered in FFPE specimens. With coupled with downstream rare variant analysis the workflow retains high fidelity with expected VAF. This supports more reliable library generation and improved sequencing performance from archival and clinical FFPE samples.

Use SRSLY NanoPlus with Forshear™ for libraries from as low as 10ng of FFPE DNA (DIN 3-9)

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SRSLY™ supports methylation sequencing workflows by enabling NGS library preparation from methyl-converted inputs. Particularly, with bisulfite-converted DNA, SRSLY captures the highly fragmented DNA generated during bisulfite conversion and can work with very low input amounts. Regardless of the conversion method used, SRSLY circumvent the 2nd strand synthesis step - this enables more reliable DNA methylation analysis from limited or challenging samples.

Use our SRSLY NanoPlus or PicoPlus Uracil+ kit with Uracil-tolerant Polymerase for general Methyl-Seq applications from cfDNA and FFPE along with your choice of methyl-conversion kits.

Contact us to learn about our NEW! SRSLY MethylPlus Product that allows both fragmentomics and methylomics from cfDNA

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Traditional bisulfite based methyl conversion methods result in spurious fragmentation of cfDNA and double-stranded library preparation methods (dsPreps) lose critical information at cfDNA native termini.

When combined with SRSLY library preparation followed by enzymatic methyl-conversion - captures both fragmentomics and methylation signals along the entire cfDNA fragment. By enabling robust capture of cfDNA methylation patterns and fragmentomic features, SRSLY™ supports early cancer detection research and advanced epigenetic biomarker discovery.

Use our SRSLY MethylPlus kit with Methylated Adapters and Uracil-tolerant Polymerase for Methyl-Seq with cfDNA (250pg - 50ng) and other inputs where native termini retention is critical.

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SRSLY™ is well suited for NGS library preparation from ancient DNA samples, which are typically ultra-low input and highly degraded. Its chemistry supports efficient library construction from short DNA fragments while minimizing sample loss. This enables researchers to generate sequencing libraries from irreplaceable ancient DNA samples with greater confidence.

Use SRSLY PicoPlus for NGS library preparation from highly degraded and limited quantity ancient DNA samples as low as 250 pg and <150bp in size

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Phospharamidite synthesis and other methods of oligosynthesis can generate truncated molecules or may have contamination. SRSLY™ supports NGS based evaluation of synthetic oligonucleotides, characterization of oligosynthesis workflows synthesis errors and in CRISPR oligo QC.

Capture the purity of your oligos as low as 20bp using the SRSLY PicoPlus Kit.

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REALLY™ enables efficiency RNA-Seq library preparation. Its high conversion rates supports reliable measurement of low-input and partially degraded RNA, including challenging samples such as FFPE-derived RNA. By providing consistent metrics regardless of the input quality REALLY™ helps improve reproducibility and confidence in RNA-Seq workflows. Use your favorite PolyA mRNA enrichment workflow or ribodepletion modules upstream or use our rNONE and hg-rNONE depletion modules for streamlined enrichment of informative transcripts.

Use our REALLY-rNONE kit for RNA-Seq from degraded RNA of RIN 3-5, as low as 10ng inputs.

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