Xactly DNA Overhang profiling
Exact ends of fragmented DNA molecules tell a story
XACTLY provides comprehensive information about the native-state of fragmentary DNA termini including the type (5´, 3´ , or blunt), length, and sequence of all single-stranded overhangs. The generation of blunt ends or overhanging termini can result from physical, chemical, or enzymatic cleavage, physiologic processes or pathological mechanisms. The native termini of DNA molecules reveal information about where they have been and what they have interacted with.
We initially developed XACTLY to interrogate cell-free DNA degradation. While our internal cfDNA research is ongoing, we’ve also found many other exciting applications for XACTLY including: identifying restriction enzyme cut sites, nuclease digestion preferences, and better understanding DNA degradation over time.
XACTLY works by ligating a pool of end-informative NGS adapters to substrate DNA without the conventional end-polishing step. By incorporating different Unique End Identifiers (UEI) for adapters of each length and type, XACTLY encodes the exact type, length and overhang sequence, if present, for every DNA molecule in a massively parallelized reaction. The result is a standard dsDNA sequencing library that adds novel information about the fragment termini.
Xactly in Practice
OUr Vision for XACLTY
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While XACTLY is not currently publicly available, we are always looking for research collaborations and partnerships. Please contact us if XACTLY sounds right for your research question! And check back for updates on our ongoing research with XACTLY. In the meantime check out our current literature.