cfDNA | FFPE-DNA | MEthyl-seq | RNA-Seq | Ancient DNA | OLIGO QC
Click on each application to learn more.
KEY DATA HIGHLIGHTS
Captures nicked, ssDNA and damaged DNA
Retains native ends
Captures short DNA between 20-50bp
Generates libraries of higher complexity for the same input amount
Higher concordance between expected and observed variant calls, especially for degraded inputs (DIN 3)
PRODUCT SPECIFICATIONS
Sequence-ready library in under 3 hours
Few PCR cycles required - reduce sequence biases
Compatible with multiple input types
Complete kit with reagents for adapter ligation, indexing PCR and magnetics beads for purification
Optional: ForShear for Enzymatic Shearing for application with FFPE
Optional: UMI-Add on for rare-variant analysis
ONE KIT MANY APPLICATIONS
As you know, degraded DNA is a very common challenge in NGS workflows. A variety of sample types yield low quality DNA – FFPE tissue, liquid biopsy and rootless hair. These DNA fragments could be short (<100 bp), damaged, nicked or single-stranded and in low amounts (< 1ng). Most library preparation methods are unable to capture all the DNA molecules from such sources.
Launched in 2019, SRSLY library kit from ClaretBio is a single-stranded library preparation method that offers a simple, fast, easily automatable way to convert DNA from challenging inputs into sequencing libraries of high complexity. We have developed additional modules ForShear™, rNONE™ and UMI Add-on to expand the application of our workflow to a broader range of sample types.
Tired of switching NGS kits based on sample quality or quantity? Let us take the guesswork out of it, SRSLY!