Claret Bioscience® and New England Biolabs® Announce Co-Promotion Collaboration for Methylation and Fragmentomics Research-Use Workflows

Claret Bioscience and New England Biolabs (NEB® ) have entered into a co-promotion agreement to support researchers using SRSLY® MethylPlus in combination with the NEBNext® EM-seq™ v2 Conversion Module.

SRSLY MethylPlus and SRSLY Uracil+ products are designed to integrate with enzymatic methyl-conversion workflows, enabling high-quality library preparation for methylation profiling and fragmentomics research. The collaboration with NEB provides researchers with a validated workflow option that combines SRSLY MethylPlus library construction with EM-seq v2 conversion chemistry.

This combined workflow supports research with:

• Whole-genome methylation analysis

• Targeted methylation studies

• cfDNA Fragmentomics research

• Broader epigenomic applications

SRSLY-based workflows are designed for compatibility with diverse methyl-conversion workflows, enabling researchers to select configurations best suited to their applications. The SRSLY MethylPlus workflow works with inputs as low as 100pg to 50ng for downstream methyl-conversion.

The NEBNext® EM-seq™ v2 Conversion Module  is an enzyme-based method for the identification of 5mC and 5hmC from as little as 100 picograms of input DNA. According to Teni Dowdell, Corporate and Business Development Manager at New England Biolabs, “We’re excited for this partnership to demonstrate the capabilities of Enzymatic Methyl-seq in conjunction with SRSLY single-stranded library preparation”

For additional technical information or workflow guidance, please contact our teams.

About ClaretBio

Claret Bioscience develops cutting-edge nucleic acid library preparation kits tailored for fragmented and degraded samples. Their patented SRSLY® technology enables ultra-efficient single-stranded DNA and RNA library prep, optimized for applications ranging from cfDNA to ancient DNA and FFPE-derived material. ClaretBio manufactures the kits under ISO13485 certified manufacturing facilities in Scotts Valley, California.

SRSLY® and SRSLY® MethylPlus are registered trademarks of Claret Bioscience LLC.

About New England Biolabs

For over 50 years, New England Biolabs (NEB) has pioneered the discovery and production of innovative products tailored for molecular biology research. Our commitment to scientific discovery is evident in all that we do, including our ever-expanding product portfolio, investment in our basic and applied research program, and support of customers' research in academia and industry, including cutting-edge technologies for use in molecular diagnostics and nucleic-acid vaccines development. Guided by our founding principles, NEB proactively invests in efforts to improve the well-being of our employees, surrounding communities, as well as the future of our planet. NEB remains a privately held company with global reach, supported by our headquarters in Ipswich, MA, USA, subsidiary offices in 11 countries, and over 60 distribution partners around the world. For more information about New England Biolabs, visit www.neb.com.

NEW ENGLAND BIOLABS®, NEB®, and NEBNEXT® are registered trademarks of New England Biolabs, Inc.

AUTOMATION PARTNERSHIP WITH HAMILTON COMPANY

ClaretBio NGS library preparation workflows SRSLY and REALLY are now supported on the Hamilton Company NGS Star line of instruments. Read the press release here: https://www.hamiltoncompany.com/press-releases/claret-bioscience-and-hamilton

Visit www.claretbio.com/automation to learn more about the other systems that ClaretBio prep can be run on

SRSLY Methodology Publication

Background

Cell-free DNA (cfDNA), present in circulating blood plasma, contains information about prenatal health, organ transplant reception, and cancer presence and progression. Originally developed for the genomic analysis of highly degraded ancient DNA, single-stranded DNA (ssDNA) library preparation methods are gaining popularity in the field of cfDNA analysis due to their efficiency and ability to convert short, fragmented DNA into sequencing libraries without altering DNA ends. However, current ssDNA methods are costly and time-consuming.

Results

Here we present an efficient ligation-based single-stranded library preparation method that is engineered to produce complex libraries in under 2.5 h from as little as 1 nanogram of input DNA without alteration to the native ends of template molecules. Our method, called Single Reaction Single-stranded LibrarY or SRSLY, ligates uniquely designed Next-Generation Sequencing (NGS) adapters in a one-step combined phosphorylation/ligation reaction that foregoes end-polishing. Using synthetic DNA oligos and cfDNA, we demonstrate the efficiency and utility of this approach and compare with existing double-stranded and single-stranded approaches for library generation. Finally, we demonstrate that cfDNA NGS data generated from SRSLY can be used to analyze DNA fragmentation patterns to deduce nucleosome positioning and transcription factor binding.

Conclusions

SRSLY is a versatile tool for converting short and fragmented DNA molecules, like cfDNA fragments, into sequencing libraries while retaining native lengths and ends.